Application
Sample (30 ug of whole cell lysate) A: BCL-1 10% SDS PAGE diluted at 1:2000Application
eIF3e antibody detects EIF3E protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: EIF3E protein stained by eIF3e antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using eIF3e antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using eIF3e antibody at 1:500 dilution.| Product Name | eIF3e antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF3e (eukaryotic translation initiation factor 3, subunit E)Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF3e. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus Monkey, BovineXenopus laevis (97%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF3-P48 antibody, EIF3S6 antibody, INT6 antibody, eIF3-p46 antibody, EIF3E antibody, murine mammary tumor integration site 6 (oncogene homolog) antibody, eIF-3 p48 antibody, eukaryotic translation initiation factor 3 subunit 6 antibody, viral integration |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: BCL-1 10% SDS PAGE diluted at 1:2000
Application
eIF3e antibody detects EIF3E protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: EIF3E protein stained by eIF3e antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 10% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using eIF3e antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using eIF3e antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
