Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000Application
EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3K protein stained by EIF3K antibody [N1C2] diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | EIF3K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eIF3K (eukaryotic translation initiation factor 3, subunit K)The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF3K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Zebrafish (88%), Xenopus laevis (93%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF3-p28 antibody, EIF3S12 antibody, HSPC029 antibody, M9 antibody, MSTP001 antibody, PLAC-24 antibody, PLAC24 antibody, PRO1474 antibody, PTD001 antibody, EIF3K antibody, eukaryotic translation initiation factor 3 subunit 12 antibody, muscle specific anti |
|---|---|
| Target | EIF3K |
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000
Application
EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3K protein stained by EIF3K antibody [N1C2] diluted at 1:500. Blue: Hoechst 33343 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
