Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using EIF4B antibody at 1:500 dilution.Application
EIF4B antibody detects EIF4B protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000Application
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000Application
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.Application
EIF4B antibody [N1N2], N-term detects EIF4B protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7.5% SDS PAGE diluted at 1:1000| Product Name | EIF4B antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF4B (eukaryotic translation initiation factor 4B)Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human EIF4B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (97%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF-4B antibody PRO1843 antibody EIF4B antibody eukaryotic translation initiation factor 4B antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using EIF4B antibody at 1:500 dilution.
Application
EIF4B antibody detects EIF4B protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000
Application
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000
Application
EIF4B antibody detects EIF4B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.
Application
EIF4B antibody [N1N2], N-term detects EIF4B protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , MCF-7 , U87-MG , SK-N-SH , SK-N-AS , 293T , A431 , HeLa , HepG2 , human EIF4B-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
