Application
ApplicationApplication
Western Blot: eIF4G1 Antibody - Rat liver lysate [10K x g supernatant, approx. 50 ug (left) or 150 ug (right lane)] separated on 7.5% gel and transferred to PVDF membranes.| Product Name | EIF4G1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to eIF4G1eIF-4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4, which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Human eIF4-gamma migrates as a cluster of polypeptides in the range of 200 to 220 kDa during SDS-PAGE, raising the possibility that it is a family of proteins encoded by separate genes (1) |
| Immunogen | Synthetic peptide (Human) - which represents a portion of human eukaryotic translation InitiationFactor 4 Gamma 1 encoded within amino acid residues 575-625. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000 - 1/10000. Please note that eIF4G breaks down very quickly. If multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | EIF4G p220 1981 EIF4G1 Q04637 600495 EIF4F DKFZp686A1451 |
|---|---|
| Target | EIF4G1 |
Application
Application
Application
Western Blot: eIF4G1 Antibody - Rat liver lysate [10K x g supernatant, approx. 50 ug (left) or 150 ug (right lane)] separated on 7.5% gel and transferred to PVDF membranes.| Positive Control | Rat liver lysate |
|---|---|
| Application Notes | IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000 - 1/10000. Please note that eIF4G breaks down very quickly. If multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide |
