Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using EIF5A2 antibody at 1:500 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using EIF5A2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 15% SDS PAGE diluted at 1:1000| Product Name | EIF5A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF5A2 (eukaryotic translation initiation factor 5A-2)mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF5A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Chicken (91%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF-5A2 antibody, eIF5AII antibody, EIF5A2 antibody, eukaryotic translation initiation factor 5A-2 antibody, eukaryotic initiation factor 5A antibody, eIF-5A-2 antibody, eukaryotic translation initiation factor 5A2 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus (By similarity) , Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) , Nucleus , nuclear pore complex (By similarity) |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using EIF5A2 antibody at 1:500 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using EIF5A2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 15% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
