Application
WB analysis of extracts from various cell lines using eIF6 antibody.Application
ICC/IF analysis of MCF7 cells using eIF6 antibody (green) and DAPI (blue).Application
ICC/IF analysis of A549 cells using eIF6 antibody (green) and DAPI (blue).| Product Name | eIF6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eIF6Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
| Immunogen | Recombinant protein of human EIF6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAB , b(2)gcn , ITGB4BP , p27BBP , p27(BBP) , EIF6 , eIF-6 , eukaryotic translation initiation factor 6 , EIF3A |
|---|---|
| Target | eIF6 |
| Molecular Weight(MW) | 27 kDa (note) |
Application
WB analysis of extracts from various cell lines using eIF6 antibody.
Application
ICC/IF analysis of MCF7 cells using eIF6 antibody (green) and DAPI (blue).
Application
ICC/IF analysis of A549 cells using eIF6 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
