Application
ELAC2 antibody detects ELAC2 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ELAC2 antibody diluted at 1:500.Application
Immunoprecipitation of ELAC2 protein from Jurkat whole cell extracts using 5 μg of ELAC2 antibody . Western blot analysis was performed using ELAC2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELAC2 antibody diluted at 1:1000.| Product Name | ELAC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ELAC2 (elaC ribonuclease Z 2)The protein encoded by this gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity, which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELAC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | elaC homolog 2 (E. coli) , ELC2 , HPC2 |
|---|---|
| Target | ELAC2 |
| Molecular Weight(MW) | 92 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
ELAC2 antibody detects ELAC2 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ELAC2 antibody diluted at 1:500.
Application
Immunoprecipitation of ELAC2 protein from Jurkat whole cell extracts using 5 μg of ELAC2 antibody . Western blot analysis was performed using ELAC2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELAC2 antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
