Application
Western blot analysis of extracts from 293 and HT29 cells using Elk1 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 AntibodyApplication
Immunofluorescence staining of methanol-fixed Hela cells using Elk1 Antibody .| Product Name | ELK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Elk1Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene |
| Immunogen | Peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ELK1, ETS-like gene 1, ETS domain-containing protein Elk-1, ELK1, member of ETS oncogene family |
|---|---|
| Target | Elk1 |
| Molecular Weight(MW) | 62 (note) |
Application
Western blot analysis of extracts from 293 and HT29 cells using Elk1 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 Antibody
Application
Immunofluorescence staining of methanol-fixed Hela cells using Elk1 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
