Application
Western blot of ELK1 antibody using| Product Name | ELK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ELK1The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. |
| Immunogen | Recombinant full length Elk1 protein (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC-P: Use at a concentration of 20 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 45 kDa (observed band size: 62 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 311040, ELK1, 2002, P19419 |
|---|---|
| Target | ELK1 |
| Tissue Specificity | recognises Elk1. |
| Cellular Localization | Nuclear |
Application
Western blot of ELK1 antibody using| Application Notes | IHC-P: Use at a concentration of 20 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 45 kDa (observed band size: 62 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Thimerosal (merthiolate). Constituents: 50% Glycerol, 1% BSA, PBS. |
