Application
Western blot analysis of NET (pS357) expression in HUVEC PMA-treated (A); HT29 PMA-treated (B) whole cell lysates.Application
Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | ELK3 (phospho Ser357) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ELK3 (phospho Ser357)This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NET (pS357). The exact sequence is proprietary. |
| Modification | p-Ser357 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ELK3, ETS-domain protein (SRF accessory protein 2) , SAP2 , ERP , ELK3 , SAP-2 , NET |
|---|---|
| Target | ELK3 (phospho Ser357) |
| Tissue Specificity | Recognizes endogenous levels of NET (pS357) protein. |
Application
Western blot analysis of NET (pS357) expression in HUVEC PMA-treated (A); HT29 PMA-treated (B) whole cell lysates.
Application
Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
