Application
ELK3 antibody [N1C2] detects ELK3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELK3 protein stained by ELK3 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ELK3 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ELK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ELK3 (ELK3, ETS-domain protein (SRF accessory protein 2))The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (91%), Rat (92%), Chicken (88%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERP antibody, NET antibody, SAP2 antibody, ELK3 antibody, SAP-2 antibody, ETS domain-containing protein Elk-3 antibody, ETS-related protein ERP antibody, serum response factor accessory protein 2 antibody, ETS-domain protein antibody, SRF accessory protein |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
ELK3 antibody [N1C2] detects ELK3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELK3 protein stained by ELK3 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ELK3 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
