Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ELP2 antibody at 1:250 dilution.Application
ELP2?antibody immunoprecipitates ELP2?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of ELP2?protein by 2 μg of ELP2?antibody 7.5% SDS-PAGE The immunoprecipitated ELP2?protein was detected by ELP2?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ELP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (20 ug ) A: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000| Product Name | ELP2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ELP2 (elongation protein 2 homolog (S. cerevisiae))Regulates the ligand-dependent activation of STAT3. Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (82%), Rat (82%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10879 antibody, SHINC-2 antibody, STATIP1 antibody, StIP antibody, ELP2 antibody, signal transducer and activator of transcription 3 interacting protein 1 antibody, signal transducer and activator of transcription interacting protein 1 antibody, elongat |
|---|---|
| Molecular Weight(MW) | 93 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ELP2 antibody at 1:250 dilution.
Application
ELP2?antibody immunoprecipitates ELP2?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of ELP2?protein by 2 μg of ELP2?antibody 7.5% SDS-PAGE The immunoprecipitated ELP2?protein was detected by ELP2?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ELP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (20 ug ) A: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
