Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using ELP4 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using ELP4 antibody at 1:250 dilution.Application
ELP4 antibody immunoprecipitates ELP4 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of ELP4 protein by 2 μg of ELP4 antibody 10% SDS-PAGE The immunoprecipitated ELP4 protein was detected by ELP4 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Product Name | ELP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ELP4 (elongation protein 4 homolog (S. cerevisiae))This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C11orf19 antibody, FLJ20498 antibody, PAX6NEB antibody, PAXNEB antibody, dJ68P15A.1 antibody, ELP4 antibody, PAX6 neighbor gene protein antibody, elongator complex protein 4 antibody, hELP4 antibody, elongation protein 4 homolog (S. cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using ELP4 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using ELP4 antibody at 1:250 dilution.
Application
ELP4 antibody immunoprecipitates ELP4 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of ELP4 protein by 2 μg of ELP4 antibody 10% SDS-PAGE The immunoprecipitated ELP4 protein was detected by ELP4 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
