Application
Western blot analysis of EMD antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EMD (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of MDA-231 cells using EMD Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Emerin antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EMD (C-term)Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 193~222 amino acids from the C-terminal region of human EMD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | EMD, Emerin, STA, EDMD |
|---|---|
| Target | EMD |
| Molecular Weight(MW) | 28.994 kDa (note) |
| Cellular Localization | Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify |
Application
Western blot analysis of EMD antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EMD (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of MDA-231 cells using EMD Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
