Application
Western blot analysis of EMILIN1 Antibody (N-term) in K562 and 293 cell line lysates (35ug/lane). EMILIN1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using EMILIN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EMILIN1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EMILIN1 (N-term)This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 91~120 amino acids from the N-terminal region of human EMILIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Elastin microfibril interfacelocated protein1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1, EMILIN-1, Elastin microfibril interfacelocated protein 1, EMILIN1, Elastin microfibril interfacer1, EMI, EMILIN 1 |
|---|---|
| Target | EMILIN1 |
| Molecular Weight(MW) | 106.667 kDa (note) |
| Cellular Localization | Found mainly at the interface between amorphous elastin and microfibrils. |
Application
Western blot analysis of EMILIN1 Antibody (N-term) in K562 and 293 cell line lysates (35ug/lane). EMILIN1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using EMILIN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
