Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE EML1 antibody diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1 antibody at 1:500 dilution.Application
EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. EML1 antibody [C3], C-term dilution: 1:500.Application
EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EML1 antibody [C3], C-term dilution: 1:500.Application
EML1 antibody [c3], c-term detects EML1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EML1 protein stained by EML1 antibody [c3], c-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Product Name | EML1 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EML1 (echinoderm microtubule associated protein like 1)Human echinoderm microtubule-associated protein-like is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EML1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseMonkeyRat |
| Predicted Reactivity | ZebrafishZebrafish (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ELP79 antibody, EMAP antibody, EMAPL antibody, FLJ45033 antibody, HuEMAP antibody, EML1 antibody, huEMAP-1 antibody, echinoderm microtubule-associated protein-like 1 antibody, EMAP-1 antibody, echinoderm microtubule associated protein like 1 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE EML1 antibody diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1 antibody at 1:500 dilution.
Application
EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. EML1 antibody [C3], C-term dilution: 1:500.
Application
EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EML1 antibody [C3], C-term dilution: 1:500.
Application
EML1 antibody [c3], c-term detects EML1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EML1 protein stained by EML1 antibody [c3], c-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
