Application
Western blot analysis of anti-EN1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EN1(arrow) was detected using the purified Pab.Application
Fluorescent image of U251 cell stained with EN1 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).EN1 immunoreactivity is localized to Nucleus significantly.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EN1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to EN1 (Engrailed 1) (N-term) |
| Immunogen | KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human EN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:100~1:500 IHC-P:1:10~1:50 ICC/IF:1:25~1:100 |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EN1, Homeobox protein engrailed1, Homeobox protein engrailed-1, Hu-En-1, HuEn1, HME1, Hu En 1, Homeobox protein engrailed 1 |
|---|---|
| Target | EN1 (Engrailed 1) |
| Molecular Weight(MW) | 40.101 kDa (note) |
Application
Western blot analysis of anti-EN1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EN1(arrow) was detected using the purified Pab.
Application
Fluorescent image of U251 cell stained with EN1 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).EN1 immunoreactivity is localized to Nucleus significantly.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~1:500 IHC-P:1:10~1:50 ICC/IF:1:25~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
