Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:10000Application
EN2 antibody immunoprecipitates EN2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 μg of EN2 antibody 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | EN2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EN2 (engrailed homeobox 2)Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq] |
| Immunogen | Full length human EN2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (84%), Rat (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EN2 antibody, hu-En-2 antibody, engrailed-2 antibody, homeobox protein engrailed-2 antibody, engrailed homolog 2 antibody, homeobox protein en-2 antibody, engrailed homeobox 2 antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:10000
Application
EN2 antibody immunoprecipitates EN2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 μg of EN2 antibody 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
