Application
Western blot analysis of ENaC-Alpha in rat kidney tissue using a 1:1000 dilution of| Product Name | ENaC Alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ENaC AlphaThe Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in transepithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6). Selected References 1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589- 594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128. |
| Immunogen | AA46–68 of the rat sequence |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| We recommend a working dilution of 1:1000 (WB). | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Scnn1a |
|---|---|
| Target | ENaC Alpha |
| Molecular Weight(MW) | 85kDa (note) |
| Cellular Localization | Apical membrane of epithelial cells. |
Application
Western blot analysis of ENaC-Alpha in rat kidney tissue using a 1:1000 dilution of| Application Notes | We recommend a working dilution of 1:1000 (WB). |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Rabbit immunoglobulin in 50% glycerol |
