Application
Western blot analysis of extracts from HepG2 cells, using ENAH Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ENAH Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | ENAH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ENAHEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ENAH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Protein enabled homolog |
|---|---|
| Target | ENAH |
| Molecular Weight(MW) | 66 kDa (note) |
| Tissue Specificity | ENAH Antibody detects endogenous levels of total ENAH protein. |
| Cellular Localization | Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at s |
Application
Western blot analysis of extracts from HepG2 cells, using ENAH Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ENAH Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
