Application
Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at μg/ml.Application
Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G| Product Name | EndoG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EndoGThe activities of caspase and nuclease are involved in DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Studies have demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro. |
| Immunogen | Synthetic peptide: GGPRGPGELAKYGLP, corresponding to amino acids 55-70 of Human Endonuclease G. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 1 - 2 μg/ml. IHC-P: Use at a concentration of 10-15μg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa), determined using human HepG2 (hepatoblastoma) cell lysate. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Endonuclease G, 600440, Q14249, 2021, ENDOG |
|---|---|
| Target | EndoG |
| Tissue Specificity | This antibody recognizes human, mouse, and rat EndoG (Endonuclease G). |
| Cellular Localization | Mitochondrial |
Application
Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at μg/ml.
Application
Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml. IHC-P: Use at a concentration of 10-15μg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa), determined using human HepG2 (hepatoblastoma) cell lysate. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
