Rabbit Polyclonal antibody to Fas (endonuclease G)The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Fas. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouse
Predicted Reactivity
Rat, BovineRat (95%), Bovine (96%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
FLJ27463 antibody, ENDOG antibody, mitochondrial endonuclease G antibody, endonuclease G, mitochondrial antibody, endo G antibody, endonuclease G antibody
Molecular Weight(MW)
33 kDa (note) (2)
Cellular Localization
Mitochondrion
Application
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:500
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:500
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.