Application
Endomorphin-1 and 2 staining in rat paraventricular thalamic nucleus.| Product Name | Endomorphin 1 & 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Endomorphin 1 & 2Recently, endogenous agonists for the mu-opiate receptor, were first isolated from bovine brain [Nature,386, 499 (1997)]. These are endomorphin-1; Tyr-Pro-Trp-Phe-NH2, and endomorphin-2; Tyr-Pro-Phe-Phe-NH2. The isolation of relatively large amounts of endomorphin-1 and endomorphin-2 from human brain cortex has also reported [Peptides,18, 1635 (1997)].The immunoreactivity for endomorphin was also detected in rat brain near the mu-opiate receptor containing neurons, but the molecular form of the rat peptide has not yet been elucidated [Peptides,18, 1641 (1997)]. These observations may be considered to indicate that endomorphin-1 and 2 are common mu-opiate agonists in mammals. In addition to the originally discovered mu-opiate agonistic function, these peptides have been reported to show hypotensive activity and Ca2+ channel current inhibitory activity [Biochem. Biophys. Res. Commun.,235, 567 (1997)]. Endomorphins would be useful for the study of pain perception together with some other biological functions in the body. |
| Immunogen | Synthetic peptide conjugated to BSA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-F |
| ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 3-10 μg/ml. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseMonkeyPigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Endomorphin 1 &2 |
|---|---|
| Target | Endomorphin 1 & 2 |
| Tissue Specificity | Sensitivity has been determined by ELISA. Less than 1ng of antibody could be detected after binding to Endomorphin 2 which is conjugated to titer plate:Endomorphin 2 = 100Endomorphin 1 = 70Leu 5 -Enkephalin = 5-Enkephalin = |
Application
Endomorphin-1 and 2 staining in rat paraventricular thalamic nucleus.| Application Notes | ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 3-10 μg/ml. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Restore with 50µl of dH2O containing 0.1% sodium azide or other preservative |
