Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin antibody [N1C2] dilution: 1:250.IF
(B) Immunofluorescence staining: Green fluorescence indicates active CD31; red fluorescence indicates active Emcn, TRAP and OCN respectively; blue fluorescence indicates PI bound to the nuclei of cells.IHC
CD31&Emcn staining of histological sections from different groups. Green fluorescence: CD31; Red fluorescence: Emcn; Blue fluorescence: nucleus of the cells; Quantification of CD31&Emcn double positive staining of different groups, N≥ 8, p< 0.05 (Independent t-test).| Product Name | Endomucin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Endomucin (endomucin)EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Endomucin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRhesus Monkey |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | EMCN2 antibody, MUC14 antibody, EMCN antibody, endomucin-2 antibody, endomucin antibody, gastric cancer antigen Ga34 antibody, MUC-14 antibody, mucin-14 antibody |
|---|---|
| Target | Endomucin |
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein , Membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin antibody [N1C2] dilution: 1:250.
IF
(B) Immunofluorescence staining: Green fluorescence indicates active CD31; red fluorescence indicates active Emcn, TRAP and OCN respectively; blue fluorescence indicates PI bound to the nuclei of cells.
IHC
CD31&Emcn staining of histological sections from different groups. Green fluorescence: CD31; Red fluorescence: Emcn; Blue fluorescence: nucleus of the cells; Quantification of CD31&Emcn double positive staining of different groups, N≥ 8, p< 0.05 (Independent t-test).| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
