Application
Western blot analysis of EDNRA antibody (Center) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EDNRA Antibody (Center) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Endothelin A Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Endothelin A ReceptorThis gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Immunogen | synthetic peptide between 125-154 amino acids from the Central region of human EDNRA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| WB: 1:1000. IF: 1:10 - 1:50. FACS: 1:10 - 1:50 | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | ETA , ETA R , endothelin receptor type A , ET A , ETAR , ETRA , hET AR , EDNRA |
|---|---|
| Target | Endothelin A Receptor |
| Tissue Specificity | This EDNRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human EDNRA. |
Application
Western blot analysis of EDNRA antibody (Center) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EDNRA Antibody (Center) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB: 1:1000. IF: 1:10 - 1:50. FACS: 1:10 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
