Application
Western blot analysis of extracts of various cell lines, using ENO1 antibody.Application
Immunofluorescence analysis of HeLa cell using ENO1 antibody. Blue: DAPI for nuclear staining.| Product Name | ENO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENO1This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| Immunogen | Recombinant protein of human ENO1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ENO1L1 , PPH , enolase 1, (alpha) , NNE , MPB1 |
|---|---|
| Target | ENO1 |
Application
Western blot analysis of extracts of various cell lines, using ENO1 antibody.
Application
Immunofluorescence analysis of HeLa cell using ENO1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
