Rabbit polyclonal antibody to ENO1This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Immunogen
Native human Enolase 1
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHCIHC-FIHC-PWB
Immunohistology - Frozen: 1/25 - 1/100
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
MPB1 , ENO1L1 , ENO1 , PPH , NNE
Target
Enolase
Tissue Specificity
This antibody recognizes human non-neuronal enolase, also known as alpha enolase or enolase 1. It demostrates less than 0.1% cross-reactivity with neuron-specific enolase.
Application
Application
IHC ImageStaining of Jurkat cells with Rabbit anti Human NNE polyclonal antibody
Application Notes
Immunohistology - Frozen: 1/25 - 1/100
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.