Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ENO1 antibody at 1:500 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:20000.Application
Immunofluorescence analysis of methanol-fixed HCT116, using ENO1 antibody at 1:500 dilution.Application
ENO1antibody immunoprecipitates ENO1protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of ENO1protein by 2.5 μg of ENO1 antibody 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | ENO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENO1 (enolase 1, (alpha))This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigMouse (94%), Rat (94%), Xenopus laevis (92%), Chicken (92%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%), Guinea pig (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ENO1L1 antibody, MPB1 antibody, NNE antibody, PPH antibody, ENO1 antibody, non-neural enolase antibody, 2-phospho-D-glycerate hydro-lyase antibody, alpha-enolase, c-myc promoter-binding protein-1 antibody, alpha enolase like 1 antibody, tau-crystallin ant |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ENO1 antibody at 1:500 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:20000.
Application
Immunofluorescence analysis of methanol-fixed HCT116, using ENO1 antibody at 1:500 dilution.
Application
ENO1antibody immunoprecipitates ENO1protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of ENO1protein by 2.5 μg of ENO1 antibody 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
