Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 5% SDS-PAGE eNOS (phospho Ser1177) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract (untreated) B. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 6 hr) C. 30 µg THP-1 whole cell lysate/extract (untreated) D. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 24 hr) E. 30 µg THP-1 whole cell lysate/extract (untreated) F. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 48 hr) 5% SDS-PAGE eNOS (phospho Ser1177) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: eNOS (phospho Ser1177) protein stained by eNOS (phospho Ser1177) antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | eNOS (phospho Ser1177) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eNOS (phospho Ser1177) (nitric oxide synthase 3 (endothelial cell))Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Modification | p-Ser1177 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCICC/IFIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMonkeyMouseRat |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Sheep, Bovine, Guinea pigMouse (100%), Rat (100%), Dog (100%), Pig (100%), Rabbit (100%), Sheep (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NOS3, nitric oxide synthase 3 (endothelial cell), eNOS, ECNOS |
|---|---|
| Target | eNOS (phospho Ser1177) |
| Cellular Localization | Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus |
Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 5% SDS-PAGE eNOS (phospho Ser1177) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract (untreated) B. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 6 hr) C. 30 µg THP-1 whole cell lysate/extract (untreated) D. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 24 hr) E. 30 µg THP-1 whole cell lysate/extract (untreated) F. 30 µg THP-1 whole cell lysate/extract (100 nM PMA treatment for 48 hr) 5% SDS-PAGE eNOS (phospho Ser1177) antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: eNOS (phospho Ser1177) protein stained by eNOS (phospho Ser1177) antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
