Application
Western Blot analysis of eNOS in Jurkat cell lysate.| Product Name | eNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eNOSeNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cNOS, ECNOS, NOS3, EC-NOS, NOSIII, EC NOS |
|---|---|
| Target | eNOS |
| Tissue Specificity | This antibody is specific for eNOS. |
| Cellular Localization | Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
Application
Western Blot analysis of eNOS in Jurkat cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC. The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
