Application
eNOS antibody detects eNOS protein by western blot analysis. A. 30 μg THP-1 whole cell lysate/extract (untreated) B. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 6 hr) C. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 24 hr) 5 % SDS-PAGE eNOS antibody dilution: 1:1000| Product Name | eNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eNOS (nitric oxide synthase 3 (endothelial cell))Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eNOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Sheep, Bovine, Guinea pigMouse (100%), Rat (100%), Dog (100%), Pig (100%), Rabbit (100%), Sheep (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NOS3, nitric oxide synthase 3 (endothelial cell), ECNOS |
|---|---|
| Target | eNOS |
| Molecular Weight(MW) | 133 kDa (note) |
| Cellular Localization | Cell membrane , Membrane , caveola , Cytoplasm , cytoskeleton , Golgi apparatus |
Application
eNOS antibody detects eNOS protein by western blot analysis. A. 30 μg THP-1 whole cell lysate/extract (untreated) B. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 6 hr) C. 30 μg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 24 hr) 5 % SDS-PAGE eNOS antibody dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
