Application
Western blot analysis of NOS3 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). NOS3 (arrow) was detected using the purified Pab.Application
NOS3 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NOS3 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | eNOS antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NOS3 (N-term)Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 54~84 amino acids from the N-terminal region of human NOS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cNOS, Nitric oxide synthase, endothelial, Constitutive NOS, NOS type III, ECNOS, eNOS, Endothelial NOS, NOS3, EC-NOS, NOSIII, EC NOS |
|---|---|
| Target | NOS3 |
| Molecular Weight(MW) | 133.289 kDa (note) |
| Cellular Localization | Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
Application
Western blot analysis of NOS3 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). NOS3 (arrow) was detected using the purified Pab.
Application
NOS3 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NOS3 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
