Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using ENOSF1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ENOSF1 antibody at 1:500 dilution.| Product Name | ENOSF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENOSF1 (enolase superfamily member 1)This gene was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Alternate splice variants have been described, one of which (named rTSalpha) represents an alternate 3'UTR that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and down-regulates TS expression. Another transcript variant (named rTSalpha) does not overlap the TS locus. The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENOSF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Pig, BovinePig (86%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSRTSBETA antibody, RTS antibody, TYMSAS antibody, ENOSF1 antibody, rTS beta antibody, rTS alpha antibody, mitochondrial enolase superfamily member 1 antibody, antisense RNA to thymidylate synthase antibody, enolase superfamily member 1 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (4) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ENOSF1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ENOSF1 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
