Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Product Name | ENPP6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENPP6 (ectonucleotide pyrophosphatase/phosphodiesterase 6)Choline-specific glycerophosphodiester phosphodiesterase. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENPP6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (94%), Rat (95%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC33971 antibody, NPP6 antibody, ENPP6 antibody, E-NPP 6 antibody, ectonucleotide pyrophosphatase/phosphodiesterase family member 6 antibody, B830047L21Rik antibody, NPP-6 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 6 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Probable) , Secreted |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
