Application
Sample (30 ug of whole cell lysate) A: A549 15% SDS PAGE diluted at 1:1000Application
ENSA antibody detects ENSA protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ENSA antibody dilution: 1:500.Application
ENSA antibody detects ENSA protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold Methanol for 5 min. Green: ENSA protein stained by ENSA antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
ENSA antibody detects ENSA protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ENSA antibody dilution: 1:500.Application
ENSA antibody detects ENSA protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. ENSA antibody dilution: 1:500.| Product Name | ENSA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENSA (endosulfine alpha)The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENSA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (90%), Pig (98%), Chicken (92%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARPP-19e antibody, MGC4319 antibody, MGC78563 antibody, MGC8394 antibody, ENSA antibody, alpha-endosulfine antibody, endosulfine alpha antibody |
|---|---|
| Molecular Weight(MW) | 13 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A549 15% SDS PAGE diluted at 1:1000
Application
ENSA antibody detects ENSA protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ENSA antibody dilution: 1:500.
Application
ENSA antibody detects ENSA protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold Methanol for 5 min. Green: ENSA protein stained by ENSA antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
ENSA antibody detects ENSA protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ENSA antibody dilution: 1:500.
Application
ENSA antibody detects ENSA protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. ENSA antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
