Application
EV71 antibody detects EV71 protein by western blot analysis. Un-infected (-) and infected with EV71 C2 or B4 strain RD whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EV71 antibody at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EV71 antibody detects EV71 viral protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 antibody [ diluted at 1:500Application
Sample (25 µg of whole cell lysate) A: Vero cell lysate B: Vero cell lysate infected with EV-71 10% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed EV71 infected Vero cell, using EV71 antibody at 1:1000 and 1:5000 dilution.| Product Name | Enterovirus 71 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EV71 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Enterovirus 71 |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EV71 inactive virus Enterovirus 71 |
|---|
Application
EV71 antibody detects EV71 protein by western blot analysis. Un-infected (-) and infected with EV71 C2 or B4 strain RD whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EV71 antibody at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EV71 antibody detects EV71 viral protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 antibody [ diluted at 1:500
Application
Sample (25 µg of whole cell lysate) A: Vero cell lysate B: Vero cell lysate infected with EV-71 10% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed EV71 infected Vero cell, using EV71 antibody at 1:1000 and 1:5000 dilution.| Positive Control | Vero cell lysate infected with EV-71 , EV71 C2 infected RD , EV71 B4 infected RD |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
