Application
Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EV71 VP3 antibody diluted at 1:1000.Application
EV71 VP3 antibody detects EV71 VP3 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP3 protein stained by EV71 VP3 antibody diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.| Product Name | Enterovirus 71 VP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EV71 VP3 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of EV71 Vp3 protein. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Enterovirus 71 |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | EV71 VP3 |
|---|
Application
Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EV71 VP3 antibody diluted at 1:1000.
Application
EV71 VP3 antibody detects EV71 VP3 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% paraformaldehyde. Green: EV71 VP3 protein stained by EV71 VP3 antibody diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.| Positive Control | EV71-infected RD cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
