Application
Western blot analysis of ENTH Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ENTH antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ENTH (Internal)This gene encodes a protein with similarity to the epsin family of endocytic adapter proteins. The encoded protein interacts with clathrin, the adapter protein AP-1 and phosphoinositides. This protein may be involved in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in this gene are associated with a susceptibility to schizophrenia and psychotic disorders. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 228~258 amino acids from the Center region of human ENTH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Epsin-rel, KIAA0171, Epsinrel, ENTH, Enthoprotin, Epsinrelated protein, EPNR, Epsin4, Clathrin-interacting protein localized in the trans-Golgi region, EpsinR, Clathrin interactor 1, Epsin-4, CLINT1, Clathrin interactor1, Clathrininteracting protein locali |
|---|---|
| Target | ENTH |
| Molecular Weight(MW) | 68.259 kDa (note) |
| Cellular Localization | Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network. |
Application
Western blot analysis of ENTH Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
