Application
Immunoprecipitation of ENY2 protein from NT2D1 whole cell extracts using 5 μg of ENY2 antibody . Western blot analysis was performed using ENY2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ENY2 antibody detects ENY2 protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract C. 30 µg U87-MG whole cell lysate/extract D. 30 µg SK-N-SH whole cell lysate/extract 15% SDS-PAGE ENY2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ENY2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ENY2 (enhancer of yellow 2 homolog (Drosophila))Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENY2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (85%), Xenopus laevis (93%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20480 antibody, e(y)2 antibody, ENY2 antibody, enhancer of yellow 2 transcription factor homolog antibody, enhancer of yellow 2 homolog (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of ENY2 protein from NT2D1 whole cell extracts using 5 μg of ENY2 antibody . Western blot analysis was performed using ENY2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ENY2 antibody detects ENY2 protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract C. 30 µg U87-MG whole cell lysate/extract D. 30 µg SK-N-SH whole cell lysate/extract 15% SDS-PAGE ENY2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
