Application
IHC-P analysis of human breast cancer using EpCAM antibody [VU-1D9].| Product Name | EpCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [VU-1D9] to EpCAMEpithelial Cell Adhesion Molecule (Ep CAM) is a 40kD cell surface antigen. This antigen has been identified independently by a number of groups, and it has been known by a variety of names. Several monoclonal antibodies have been raised against Ep CAM, many of which have been described as tumour specific molecules on carcinomas. Ep CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium. |
| Immunogen | Small cell lung carcinoma cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | VU-1D9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-P |
| This antibody can be used on formalin-fixed, paraffin-embedded and frozen tissue sections at a dilution of 1:50-1:60. The optimal conditions should be determined by the individual laboratory: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | TROP1 MK-1 CO171A GA7332 M4S1 EpCAM Ep-CAM 4072 EGP40 KSA hEGP-2 CO17-1A MIC18 TACSTD1 185535 hEGP2 P16422 EGP GA733-2 MK1 DIAR5 EGP-2 EGP314 ESA HNPCC8 KS1/4 MK 1 EGP 2 Ep CAM hEGP 2 EGP2 |
|---|---|
| Target | EpCAM |
| Tissue Specificity | This antibody reacts with a 40 kD cell surface glycoprotein called ESA. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
Application
IHC-P analysis of human breast cancer using EpCAM antibody [VU-1D9].| Positive Control | Breast, colon carcinoma and tonsil |
|---|---|
| Application Notes | This antibody can be used on formalin-fixed, paraffin-embedded and frozen tissue sections at a dilution of 1:50-1:60. The optimal conditions should be determined by the individual laboratory: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Purified Ab with BSA and Azide |
Data sheet for OM251651