Application
MDK Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).Application
MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of MDK Antibody (C-term) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Confocal immunofluorescent analysis of MDK Antibody (C-term)(Cat#AP12076b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | MDK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDK; MK1; NEGF2; Midkine; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MK1 NEGF2 |
|---|---|
| Alternative Names | MDK MK1 NEGF2 Midkine Amphiregulin-associated protein Midgestation and kidney protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein |
| Molecular Weight(MW) | 15585 Da |
| Function | Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity) |
| Tissue Specificity | This MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-143 amino acids from the C-terminal region of human MDK. |
| Cellular Localization | Secreted. |
| Entrez Gene | 4192 |
|---|---|
| Nucleotide Accession | NP_001012334.1 |
Application
MDK Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).
Application
MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of MDK Antibody (C-term) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Confocal immunofluorescent analysis of MDK Antibody (C-term)(Cat#AP12076b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM213464