Application
A. Western blot analysis on 1 ng of recombinant protein using anti-Midkine RabMAb (Cat. #AJ1479a), dilution 1:500.Application
B. Immunohistochemical staining of paraffin-embedded human livercarcinoma using anti-Midkine RabMAb (Cat. #AJ1479a).Application
C. Immunofluorescent staining of HeLa cells using anti-Midkine RabMAb(Cat. #AJ1479a).| Product Name | Midkine Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDK, MK1, NEGF2, Midkine, Neurite outgrowth-promoting factor 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1143Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MK1 NEGF2 |
|---|---|
| Alternative Names | MDK MK1 NEGF2 Midkine Neurite outgrowth-promoting factor 2 |
| Molecular Weight(MW) | 15585 Da |
| Function | Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues on the C-terminus of human Midkine was used as an immunogen. |
| Cellular Localization | Secreted. |
| Entrez Gene | 4192 |
|---|
Application
A. Western blot analysis on 1 ng of recombinant protein using anti-Midkine RabMAb (Cat. #AJ1479a), dilution 1:500.
Application
B. Immunohistochemical staining of paraffin-embedded human livercarcinoma using anti-Midkine RabMAb (Cat. #AJ1479a).
Application
C. Immunofluorescent staining of HeLa cells using anti-Midkine RabMAb(Cat. #AJ1479a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM213735