Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:3000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Eph Receptor A3 antibody at 1:500 dilution.| Product Name | EphA3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EphA3This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Eph Receptor A3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, BovineMouse (97%), Rat (96%), Pig (97%), Chicken (92%), Bovine (97%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EK4 antibody ETK antibody ETK1 antibody HEK antibody HEK4 antibody TYRO4 antibody EPHA3 antibody TYRO4 protein tyrosine kinase antibody ephrin type-A receptor 3 antibody eph-like tyrosine kinase 1 antibody EPH-like kinase 4 antibody tyrosine-pro |
|---|---|
| Target | EphA3 |
| Molecular Weight(MW) | 110 kDa (note) (3) |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:3000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Eph Receptor A3 antibody at 1:500 dilution.| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
