Application
Eph receptor A4 antibody detects Eph receptor A4 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Eph receptor A4 antibody diluted by 1:500.Application
Eph receptor A4 antibody [N3C2], Internal detects Eph receptor A4 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 5 μg whole cell extract of 3xFlag-human EPHA4-transfected 293T cells 5 % SDS-PAGE Eph receptor A4 antibody [N3C2], Internal dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | EphA4 antibody , Internal |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EphA4This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Eph receptor A4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus MonkeyMouse (99%), Rat (99%), Xenopus laevis (85%), Pig (99%), Chicken (93%), Rhesus Monkey (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HEK8 antibody SEK antibody TYRO1 antibody EPHA4 antibody EK8 antibody ephrin type-A receptor 4 antibody receptor protein-tyrosine kinase HEK8 antibody tyrosine-protein kinase TYRO1 antibody TYRO1 protein tyrosine kinase antibody EPH-like kinase 8 |
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| Target | EphA4 |
| Molecular Weight(MW) | 110 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Eph receptor A4 antibody detects Eph receptor A4 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with Eph receptor A4 antibody diluted by 1:500.
Application
Eph receptor A4 antibody [N3C2], Internal detects Eph receptor A4 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 5 μg whole cell extract of 3xFlag-human EPHA4-transfected 293T cells 5 % SDS-PAGE Eph receptor A4 antibody [N3C2], Internal dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 , 3xFlag-human EPHA4-transfected 293T , human brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
