Application
Sample(30μg of whole cell lysate) A: HeLaS3 B: Hep G2 C: MOLT-4 7.5% SDS PAGE diluted at 1:1000| Product Name | EphB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EphB3Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EPH receptor B3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (98%), Rat (99%), Xenopus laevis (88%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ETK2 antibody HEK2 antibody TYRO6 antibody EPHB3 antibody ephrin type-B receptor 3 antibody EPH-like tyrosine kinase-2 antibody embryonic kinase 2 antibody EPH-like kinase 2 antibody EPH-like tyrosine kinase 2 antibody tyrosine-protein kinase TYRO |
|---|---|
| Target | EphB3 |
| Molecular Weight(MW) | 110 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample(30μg of whole cell lysate) A: HeLaS3 B: Hep G2 C: MOLT-4 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
