Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ephrin B2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Product Name | Ephrin B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ephrin B2This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ephrin-B2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Pig, ChickenMouse (96%), Rat (97%), Dog (95%), Cat (95%), Pig (95%), Chicken (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPLG5 antibody HTKL antibody Htk-L antibody LERK5 antibody MGC126226 antibody MGC126227 antibody MGC126228 antibody EFNB2 antibody ephrin-B2 antibody eph-related receptor tyrosine kinase ligand 5 antibody HTK ligand antibody LERK-5 antibody lig |
|---|---|
| Target | Ephrin B2 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Membrane |
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ephrin B2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
