Mouse monoclonal antibody [AT2E5] to EPHX1Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]
Immunogen
Recombinant human EPHX1 (21-455aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AT2E5
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.:
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of mouse liver lysate (30ug) using EPHX1 antibody at a dilution of 1:1,000.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.