Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using EPLIN antibody at 1:500 dilution.| Product Name | EPLIN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EPLIN (LIM domain and actin-binding protein 1)EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EPLIN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EPLIN antibody FLJ38853 antibody MGC131726 antibody SREBP3 antibody LIMA1 antibody 1110021C24Rik antibody sterol regulatory element binding protein 3 antibody epithelial protein lost in neoplasm beta antibody LIM domain and actin-binding protein 1 |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Cell junction , focal adhesion , cytoskeleton |
Application
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EPLIN antibody at 1:500 dilution.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
