Application
Western blot analysis detecting EPO antigen (100 ng/well) using mouse anti-human EPO antibody (clone 85) at 1:1000 dilution (1 ug/mL)| Product Name | EPO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [85] to EPOHuman erythropoietin is an acidic glycoprotein hormone with a molecular mass of 34 kD. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. |
| Immunogen | Purified CHO cell-expressed recombinant human EPO. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 85 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 133170 MGC138142 EP EPO P01588 2056 |
|---|---|
| Target | EPO |
| Tissue Specificity | Does not cross react with other human cytokines tested such as IL-8, IL-16, IL-7, MCP-1, TNF-beta, GM-CSF, M-CSF, IL-1 beta, bFGF, and human serum albumin or bovine serum albumin |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
Application
Western blot analysis detecting EPO antigen (100 ng/well) using mouse anti-human EPO antibody (clone 85) at 1:1000 dilution (1 ug/mL)| Application Notes | ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: Lyophilized from: 0.01M PBS. pH 7.2 |
