Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody. The lane on the left is treated with antigen-specific peptide.
Rabbit Polyclonal antibody to Eps15 (phospho Tyr849)This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]
Immunogen
Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.
Modification
p-Tyr849
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
The antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
Application
Application
Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody. The lane on the left is treated with antigen-specific peptide.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20˚C/1 year
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.